ASSESSMENT SERUM GLUCOSE AND PLASMA GLUCOSE UNDER IDENTICAL CONDITIONS
BILLY SÁNCHEZ–JACINTO
*
Faculty of Medicine Alberto Hurtado, School of Medical Technology, Cayetano Heredia University, Lima, Peru and Medical Technology, Cayetano Heredia University, Lima, Peru.
*Author to whom correspondence should be addressed.
Abstract
According to the International Diabetes Federation, South and Central America region has the proportion of 41.9% people with diabetes who are undiagnosed. Several factors influence the stability of glucose values after collection as “in vitro” glycolysis. Aim of this study was to compare the fasting glucose levels either in plasma or in serum under identical conditions.
Methodology: Blood samples were collected in K2 EDTA and serum tubes with clot activator and both tubes were maintained in upright position for 30 min at room temperature to allow clot formation. Also, linear regression model was used for evaluate la relationship between glucose plasma and glucose serum and multiple linear regression was used and two model for obtain coefficients adjusted by hematology parameters covariates.
Results: Mean glucose in serum tube was -1.57 mg/dl than K2 EDTA tube and that was no statistically significant difference for glucose (p = 0.41) and neither clinically significant. But on the other hand, serum glucose increases by 1 mg/dl; while EDTA glucose increases by an average of 0.59 mg/dl (p<0.05).
Conclusion: Serum glucose is similar to plasma glucose when hematology parameters between the reference range.
Keywords: Serum glucose, plasma glucose, preanalytical, glycolysis
How to Cite
Downloads
References
International Diabetes Federation. IDF Diabetes Atlas, 10th Edn. Brussels, Belgium; 2021.
Available:https://www.diabetesatlas.org
Sacks D, Arnold M, Bakris G, et al. Guidelines and recommendations for laboratory analysis in the diagnosis and management of diabetes mellitus. Clin Chem. 2011;57(6): e1-e47.
Pasqualetti S, Braga F, Panteghini M. Pre-analytical and analytical aspects affecting clinical reliability of plasma glucose results. Clin Biochem. 2017;50(10-11):587-594.
Sacks DB. A1C versus glucose testing: a comparison. Diabetes Care. 2011;34(2):518-23.
Kang JG, Park CY, Ihm SH, Park SW. A Potential Issue with Screening Prediabetes or Diabetes Using Serum Glucose: A Delay in Diagnosis. Diabetes Metab J. 2016;40(5): 414-417.
Kocijancic M, Cargonja J, Delic-Knezevic A. Evaluation of the BD Vacutainer (®) RST blood collection tube for routine chemistry analytes: clinical significance of differences and stability study. Biochem Med (Zagreb). 2014;24(3):368-75.
Seclen SN, Rosas ME, Arias AJ, Huayta E, Medina CA. Prevalence of diabetes and impaired fasting glucose in Peru: report from PERUDIAB, a national urban population-based longitudinal study. BMJ Open Diabetes Res Care. 2015;3(1):e000110
Gambino R, Piscittelli J, Ackattupathil TA, et al. Acidification of blood is superior to sodium fluoride alone as an inhibitor of glycolysis. Clin Chem. 2009; 55:1019–1021.
Lippi G, Salvagno GL, Lampus S, Danese E, Gelati M, Bovo C, Montagnana M,Simundic AM. Impact of blood cell counts and volumes on glucose concentration in uncentrifuged serum and lithium-heparin blood tubes. Clin Chem Lab Med. 2018;56(12): 2125-2131.
Frank EA, Shubha MC and D’Souza CJM. Blood glucose determination: plasma or serum? J Clin Lab Anal. 2012;26:317–320.
Dimeski G, Yow KS, Brown NN. What is the most suitable blood collection tube for glucose estimation? Ann Clin Biochem. 2015;52 (Pt 2):270-5.