ANTIDIABETIC POTENTIAL OF Irvingia gabonensis ON DIABETES INDUCED MOTOR IMPAIRMENT ON ALBINO RATS CEREBELLUM
UCHEWA O. OBINNA *
Department of Anatomy, Faculty of Basic Medical Sciences, Alex Ekwueme Federal University Ndufu-Alike, Ebonyi State, Nigeria.
UGURU A. CHIMAOBI
Department of Anatomy, Faculty of Basic Medical Sciences, Alex Ekwueme Federal University Ndufu-Alike, Ebonyi State, Nigeria.
EGWU A. OGUGUA
Department of Anatomy, Faculty of Basic Medical Sciences, Alex Ekwueme Federal University Ndufu-Alike, Ebonyi State, Nigeria.
IBEGBU O. AUGUSTINE
Department of Anatomy, Faculty of Basic Medical Sciences, Alex Ekwueme Federal University Ndufu-Alike, Ebonyi State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Hyperglycemia as a life threatening disease causes motor impairments which has been ignored by researchers and clinicians. The study investigated the antidiabetic potential of Irvingia gabonensis (IG) on diabetic induced motor disorder in albino rats. Thirty rats were assigned into 6 groups of 5 rats each. Diabetes was induced by a single intra-peritoneal injection of 60 mg/kg of Streptozotocin (STZ) and confirmed after 72 hours. Blood glucose was checked at interval of 5 days for sustained hyperglycemia. Groups C, D and E were treated with100, 200 and 300 mg/kg of IG while Group F received 500 mg/kg of metformin. Motor activities were tested using string method to ascertain the role of IG on motor impairment in diabetic rats. The supernatants of homogenates were used to assay for lipid profiles namely TChol, Trig, HDL and LDL. The result showed significant decrease in TChol, LDL, triglyceride and HDL across the treated groups compared to group B (P≤0.05). Grip strength significantly decreased in group B while the extract significantly increased the grip strength in Groups C, D and E (Table 2). Limb impairment was significantly reduced in group B compared to A and increased in groups C, D and E (P≤0.05). Microscopically, group B showed structural alterations in the cerebellum with structural improvement in treated groups C, D, and E compared to group B. In conclusion, Ig have the potential to improve grip strength and limb impairment which may be useful in addressing motor complications arising from diabetes.
Keywords: Antidiabetic, hyperglycemia, grip strength, limb impairment, Irvingia gabonensis, motor activities
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Bhaskar V, Sumant SA. Antihyperglycemic and Antihyperlipidemic Activities of Root Extracts of Calotropis Procera (Ait) R.Br on streptozotocin induced diabetic rats. Jordan Journal of Biological Sciences. 2009;2:4:177–180.
Muramatsu K, Niwa M, Tamaki T, Ikutomo M, Masu Y, Hasegawa T Shimo S, Sasaki S. Effects of Streptozotocin-induced Diabetes on Motor Neurons and Muscle Spindles in Rats. Neurosci. Res. 2017;115: 21–28.
Yurii V, Lebeda MA, Orlovskya AG, Nikonenkoa GA. Early reaction of astroglial cells in rat hippocampus to streptozotocin-induced diabetes. Neuroscience Letters. 2008; 444 (2):181– 5.
Chen Z, Li L, Sun J, Ma L. Mapping the brain in type II diabetes: Voxel-based morphometry using DARTEL. Eur. J. Radiol. 2012;81:1870–1876.
Heikkila O, Lundbom N, Timonen M, Groop PH, Heikkinen S, Makimattila S. Hyperglycaemia is associated with changes in the regional concentrations of glucose and myo-inositol within the brain. Diabetologia. 2009;52:534–540.
Ahmadpour SH, Haghir H. Diabetes mellitus type 1 induces dark neuron formation in the dentate gyrus: A study by Gallyas’ method and transmission electron microscopy. Romanian Journal of Morphology and Embryology. 2011;52(2):575–579.
Gray SM, Meijer RI, Barrett EJ. Insulin regulates brain function, but how does it get there? Diabetes. 2014;63(12):3992-7.
Hernandez-Fonseca JP, Rincon J, Pedreañez A, Viera N. Arcaya JL, Carrizo E, Mosquera J. Structural and ultrastructural analysis of cerebral cortex, cerebellum, and hypothalamus from diabetic rats. Experimental Diabetes Research. 2009;329–632.
Min Y, Lowy C, Ghebremeskel K, Thomas B, Offley-Shore B, Crawford M. Unfavorable Effect of Type 1 and Type 2 Diabetes on Maternal and Fetal Essential Fatty Acid Status: a Potential Marker of Fetal Insulin Resistance. AmericanJournal of Clinical Nutrition. 2005; 82(6):1162–1168.
Lehrke M, Marx N. Diabetes Mellitus and Heart Failure. The American Journal of Cardiology. 2017;05-14.
Chang Y-P, Lee M-S, Wu D-W, Tsai J-H, Ho P-S, Lin C-HR, Chuan H-Y. Risk factors for depression in patients with Parkinson’s disease: A nationwide nested case-control study. LoS ONE. 2020;15(7):e0236443.
Radan I, Rajer E, UrSiC EB, Neubauer D, Krii$nik C, Battelino T. Motor activity during asymptomatic nocturnal hypoglycemian adolescent with type 1 diabetes mellitus. Acta Diabetol. 2004;4l:33-37.
Wong TC, Piehler KM, Kang IA, Kadakkal A, Kellman P, Schwartzman DS, et al. Myocardial Extracellular Volume Fraction Quantified by Cardiovascular Magnetic Resonance is Increased in Diabetes and Associated with Mortality and Incident of Heart Failure Admission. Eur Heart J. 2014;35:657-664.
Muramatsu K. Diabetes mellitus-related dysfunction of the Motor system. Int. J. Mol. Sci. 2020;21:7485.
Feldman EC, Stephen JE. Endocrine disorder. Text book of Veterinary internal medicine. 6 ed., Inc. Elsevier. USA. 2005; 1563-1592.
Gardoni F, Kamal A, Bellone C, et al. Effects of Streptozotocin-Diabetes on the Hippocampal NMDA Receptor Complex in Rats. J Neurochem. 2002;80(3):438–447.
Ekundayo FO, Oladipupo OA, Ekundayo EA. Studies on the effects of microbial fermentation on bush mango (Irvingia gabonensis) seed cotyledons, African Journal of Microbiology Research. 2013;7:4363-4367.
Atangana AR, Tchoundjeu Z, Foldout JM, Asaah E, Dumb M, Leakey RRB. Domestication of Irvinga gabonesis: 1 phenotypic variation in fruit and kernels in two populations from Cameroon, Agrofor. Syst. 2001;53:55-64.
Ngondi JL, Oben JE, Minka SR. The effect of Irvingia gabonensis seeds on body weight and blood lipids of obese subjects in Cameroon. 2005;4:12.
Iwu MM. Handbook of medicinal plants. BOCA Raton.CRC Press, USA. 1993;21(9):39-41.
Oluwafemi AO, Basiru OA, Babatunji EO, Adebola BO. Hematological Properties of Irvingia Gabonensis in Male Adult Rats. Journal. Pharmacology. Science Innov. 2014;3(5):434-436.
Hossam E, Jameel A, Ali M, Ahmed A, Kairy Z, Jamaan A, Ahmed R, Ibrahim M. Alhazzaand KEI. Effect of STZ-Iinduced Diabetes on Spleen of Rats: Improvement by Camel Whey Proteins. Pakistan Journal. Zoology. 2015;47(4):1109-1116.
Tariq M, Khan HA, Deeb SAl, Moutaery KAl. Neuroprotective Effect of Nicotine Against 3-Nitropropionic Acid (3-Np)-Induced Experimental Huntington’s Disease in Rats. Brain Res. Bull. 2005;67:161–168.
Yoon S, Cho H, Kim J, Lee DW, Kim GH, Hong YS, Moon S, Park S, Lee S, Lee S, et al. Brain changes in overweight/ obese and normal-weight adults with type 2 diabetes mellitus. Diabetologia. 2017;60:1207–1217.
Wasan KM, Najafi S, Wong J, Kwong M. Assessing Plasma Lipid Levels, Body Weight, and Hepatic and Renal Toxicity Following Chronic Oral Administration of Water Soluble Phytostanol Compound FM-VP4, to Gerbils, Journal of Pharmaceutical Sciences. 2001; 4(3):228–234.
Akbarzadeh A. Induction of diabetes by streptozotocin in rats. Indian Journal of Clinical Biochemistry. 2007;22 (2):60-64.
Abdulfatai O, Sanusi M, Samson O, Adam A, Okikioluwa A, Bolaji K. Cardioprotective Activities of Ethanolic Extract Root of Ageratum conyzoides on Alloxan-Induced Cardiotoxicity in Diabetic Rats. BioMed Research International. 2020;1-9.
Ogunwande AI,Matsui T, Fujise T, Matsumoto K. Alpha-Glucosidase Inhibitory Profile of Nigerian Medicinal Plants In Immobilized Assay System. Food Science and Technology Research. 2007;13:169-172.
Weilch KD, Pfister JA, Lima FG. Effect of Α7 Nicotinic Acetylcholine Receptor Agonists and Antagonists on Motor Function in Mice. Toxicology and Applied Pharmacology. 2013;266:366-374.
Ijomone OM, Olaibe OK, Biose IJ, Mba C, Umoren KE, Nwoha PU. Performance of motor associated behavioral tests following chronic nicotine administration. Annals of Neuroscience. 2014;21(2):42-46.
Kalyani RR, Metter EJ, Egan J, Golden SH, Ferrucci L. Hyperglycemia predicts persistently lower muscle strength with aging. Diabetes Care. 2015;38(1):82–90.
Eze ED, Mohammed A, Musa KY, Tanko Y, Isa AS. Effect of ethanol leaf extract of Muncuna pruriens (Fabaceae) on lipid profile in alloxan-induced diabetic Wistar rats, British Journal of Pharmacology and Toxicology. 2012;3(3):102–109.
Murali B, Upadhyaya UM, Goyal RK. Effect of Chronic Treatment with Enicostemma Littorale in Non Insulin-Dependent Diabetic (NIDDM) Rats, Journal of Ethnopharmacology. 2002;81(2):199–204.
Arvill A, Bodin L, Awil M. Effect of Short-Term Ingestion of Konjac Glucomannan on Serum Cholesterol in Healthy Men. America Journal Clinical Nutrition. 1995;61:585-9.
Vido L, Facchin P, Antonello I, et al. Childhood Obesity Treatment: Double Blinded Trial on Dietary Fibres (glucomannan) versus placebo. Padiatr Padology. 2001;28:133-6.
Kou Z, Li C, Hu J, Zhang DL, Wu ZY, Ding T, Qu J, Li H, Li YQ. Alterations in the Neural Circuits from Peripheral Afferents to the Spinal Cord: Possible Implications for Diabetic Polyneuropathy in Streptozotocin-induced type 1 Diabetic Rats. Front. Neural Circuits. 2014;8:6.
Niyomchan A, Sricharoenvej S, Lanlua P, Baimai S. Cerebellar Synaptopathy in Streptozotocin-Induced Diabetic Rats. International Journal. Morphology. 2019;37(1): 28-35.