PHARMACOLOGICAL AND ANTI CANCER ACTIVITY OF Ipomoea sepiaria METHANOLIC EXTRACT AGAINST PC-3 CELL LINE
S. AZHAGU MADHAVAN *
Deparment of Zoology & Biotechnology, A Veeriya Vandayar Memorial Sri Pushpam College, (Autonomous) Poondi, Thanjavur, 613503, Tamil Nadu, India.
P. VINOTHA
Department of Bio-Chemistry, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
V. UMA
Department of Bio-Chemistry, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
A few conventional restorative plants contain in explored concerning their enemy of corpulence likely wellsprings of is Ipomoea sepiaria Koenig ex. Roxb. The medicine is supposed in tales game plan of prescription for various accommodating properties like, love elixir and arsenic hurting, tonic, re-establishing, diuretic, laxative, uterotonic and sterility in women and leaves is shown particularly in diabetes. Phytochemical screening of different concentrates of Ipomoea sepiaria exposed the presence and non-attendance of various phytochemicals are available in ethanol remove, further examinations were done with leaves of Ipomoea sepiaria ethanol separate. PC3 (PC-3) is a human prostate malignancy cell lines that are profoundly utilized in examining the biochemical changes prostatic disease cells. Explores on PC-3 human prostate malignancy cell lines control are required in the current many years. The anticancer action of fluid concentrate of Ipomoea sepiaria was researched by 3-(4,5-dimethylthiazol-2-yl)- 2, 5-diphenyltetrazolium (MTT) measure utilizing PC-3 cell line. The current experimentation was indicated that watery concentrate of Ipomoea sepiaria, when exposed to various focuses on PC-3 cells demonstrated IC50 cell hindrance at about 5μM for 48 hours and about 2 μM for 72 hours. The information made by this particular examination gives relevant pharmacognostic and phytochemical data needed for proper distinctive confirmation and check of leaves of this particular species.
Keywords: Fluorescence, Ipomoea sepiaria, inorganic elements, phytochemicals, PC-3 human prostate cancer cell lines.
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Sayani Majumder B, Ashok K, Nishteswar K. Evaluation of intestinal transit time of root and leaves of Ipomea sepiaria Ayu. 2013;34(4): 430–432.
Cao YH. Adipose tissue angiogenesis as a therapeutic target for obesity and metabolic diseases. Nature Reviews Drug Discovery. 2010;9(2):107-115.
Wilkinson A, Bian L, Khalil D, Gibbons K, Wong PF. Type 1 diabetic children and siblings share a decrease in dendritic cell and monocyte numbers but are differentiated by expansion of CD4+T Cells Expressing IL- 17. J Clin Cell Immunol. 2011;S2:1.
Caterson ID, Finer N. Emerging pharmacotherapy for treating obesity and associated cardio metabolic risk. Asia Pac J Clin Nutr. 2006;15:55-62.
Brito AR. How to study the pharmacology of medicinal plants in underdeveloped countries. J Ethnopharmacol. 1996;54(2-3):131-8.
Mashek DG, Khan SA, Sathyanarayan A, Ploeger JM, Franklin MP. Hepatic lipid droplet biology: Getting to the root of fatty liver. Hepatology. 2015;62: 964-967.
Marilena Meira, Eliezer Pereira da Silva, Jorge M. David. Review of the genus Ipomoea: traditional uses, chemistry and biological activities. Brazilian J. of Pharmacog. 2012;22(3):682-713.
Khandelwal KR. Practical pharmacognosy techniques and experiments. New Delhi: Nirali Prakashan. 2002;15-163.
Kokate CK. Practical Pharmacognosy. 1st ed. New Delhi: Vallabh Prakashan. 2005;111.
Rao. Effect of Pterocarpus santalinus bark, on blood glucose, serum lipids, plasma insulin and hepatic carbohydrate metabolic enzymes in streptozotocin-induced diabetic rats, Food and Chemical Toxicology. 2010;48:1281–1287.
Maruthupandian A, Mohan VR. GC-MS analysis of ethanol extract of Wattakaka volubilis (L.f) Stapf. Leaf. Int Phytomed. 2011;3:59-62.
Andrews S, Azhagu Madhavan S, Ganesan S, Arjun P, Jeyaprakash R. Baskara Sanjeevi S, Ramasamy M. Different bioactive constituents and biochemical composition of brown seaweed Spatoglossum marginatum. Waffen-Und Kostumkunde Journal. 2020;XI(IV).
ISSN NO: 0042-9945.
Vardharajulu SRM, Panagal M. Molecular docking identification of best drug molecule from Ipomoea sepiaria (Koenig Ex. Roxb.) leaves against type 2 Diabetes Mellitus. International Journal of Current. Biotechnology. 2016;4(4):7-12.
Venkataswamy R, Mubarack HM, Doss A, Ravi TK, Sukumar M. Ethnobotanical study of medicinal plants used by Malasar tribals in Coimbatore District of Tamil Nadu (South India) Asian J Exp Biol Sci. 2010;1: 387–92.
Popovic Z, Matic R, Bojovic S, Stefanovic M, Vidakovic V. Ethnobotany and herbal medicine in modern complementary and alternative medicine. An overview of publications in the field of I and C medicine 2001-2013. Journal of Ethnopharmacology. 2016;2:182-192.
Kumaradevan G, Damodaran R, Mani P, Dineshkumar G, Jayaseelan T. Phytochemical screening and GC-MS analysis of bioactive components of ethanol leaves extract of Clerodendrum phlomidis (L.). American Journal of Biological and Pharmaceutical Research. 2015;2(3):142-148.
Cotterill JA, Cunliffe WJ, Williamson B, Bulusu L. Age and sex variation in skin surface lipid composition and sebum excretion rate. Br. J. Dermatol. 1972;87(4):333–340.
Meena K. Cheruvathur, Jyothi Abraham, Dennis Thomas T. In vitro micropropagation and flowering in Ipomoea sepiaria Roxb. An important ethanomedicinal plant. Asian Pacific Journal of Reproduction. 2015;4(1): 49-53.
Shubhangi Nagorao Ingole. Phytochemical analysis of leaf extract of Ocimum americanum L. (Lamiaceae) by GCMS method. World Scientific News. 2016;37:76-87.
Meesala S, Rentala S, Kaladhar DSVGK. Anti-cancer activity of leaf extract preparation from Ipomoea sepiaria against PC-3 cell line. Int. J. Life. Sci. Scienti. Res. 2017;3(5):1295-1299. DOI: 10.21276/ijlssr.2017.3.5.5