Asian Journal of Advances in Medical Science

To test the effect of selenium & zinc in aflatoxin B1 poisoning in broiler, day-old broiler chicks (n=80) were divided into 5 treatment groups with 16 birds each, (T0-control; T1-50ppb AFB1 T2-100ppb AFB1, T3-200ppb AFB1, T4-400ppb AFB1), after treating  3 weeks, 8 birds were sacrificed & rest of the birds continued with AFB1 & started a new treatment with Zn & Se supplements at the dose rate Zn (40, 60, 80 mg/kgbwt) and Se (30, 40, 50 µg/kgbwt) respectively  in T2, T3 & T4  from 3^rd week to 5^th  week. Experiment was conducted from day 1 to 35 days of age. The broilers were weighed, bled, killed by cervical dislocation .Body weights were significantly decreased (643.66±3.17) by 400ppb aflatoxin B1 (P < 0.05) at 21 days compare to control group T0. Higher concentration aflatoxin B1 (400ppb) in T4 group significantly decreased body weight from T1 (50ppb). Aflatoxin B1 induced a significant increase biochemical parameters such as serum alanine transaminase (ALT) & aspartate transaminase (AST).Significantly elevated level (P < 0.05)  of ALT & AST were observed in T4 (58.2±0.7 & 1185±17) respectively. In case of hematological parameters, the study revealed that it also reduced the level of total erythrocyte count (TLC), total leucocyte count (TLC), hemoglobin, lymphocyte, monocyte & basophil, however it accelerated neutrophil & eosinophil level. Aflatoxin B1 raised weight of liver, spleen & diminished weight of pancreas. Higher concentration extended mortality rate upto 15% in T4 group at 3 weeks. After treating with Zn& Se, growth performances had been improved gradually, it depressed these negative effects of AFB1 in broiler. These effects of AFB1 were ameliorated by supplementation of Zn & Se. It was concluded that aflatoxin contamination (50, 100, 200, 400 ppb) in broiler diet impaired the performance in terms of body weight gain, biochemical, hematological parameter, relative organ weights & livability percentage. Supplementation of zinc & Se in the aflatoxin contaminated diet improved the adverse effects of aflatoxicosis on performance of the broiler chickens. Supplementation. In conclusion, the results demonstrated that mineral treatment (Zn & Se) might be used for correction of aflatoxin B1 in broiler.