EFFICACY OF ANTI-BACTERIAL AND ANTI-FUNGAL ACTION ON THREE MEDICINAL PLANTS EXTRACT THE Rosa gallica, Psidium guajava AND Vitis vinifera AGAINST Streptococcus mutans AND Candida albicans -AN In-vitro STUDY
SUNAYANA MANIPAL *
Department of Public Health Dentistry, Jawaharlal Nehru Institute of Medical sciences, Imphal East, India.
SAJID HUSSAIN
Department of Periodontology, Sree Balaji Dental College and Hospital, Chennai, 6001004, India.
TH SOMORENDRO SINGH
Department of Conservative Dentistry, Jawaharlal Nehru Institute of Medical Sciences, Imphal East, India.
. VENKAT
Department of Prosthodontics, SRM Dental College and Hospital, Ramapuram-600089, Chennai, Tamil Nadu, India.
*Author to whom correspondence should be addressed.
Abstract
Introduction: World health organization has been an initiative for the widespread practice of herbal medicine been practiced in developing countries and created a scientific basis to treat infection.
Aim: The aim of the study was to test the anti-bacterial and anti-fungal activity of three medical plant extract the Rosa gallica, Psidium guajava L and Vitis vinifera against the streptococcus Mutans and Candida albicans organisms.
Settings and Design: An in-vitro study was conducted collaborated with the department of microbiology and the Department of Public Health Dentistry in SRM Dental College and Hospital for a period of four months. The extract of medical plant of Rosa gallica, Psidium guajava L and Vitis vinifera was tested in different concentration to find out the effective anti-bacterial and anti-fungal action.
Methods and Materials: Extract obtained from the medicinal plant of Rosa gallica, psidium guajava L and Vitis vinifera was procured and powdered. The minimum inhibitory concentration of the obtained extracts was determined by using the tube dilution method and the bacterial strains of Streptococcus mutans and Candida albicans was cultured into the extract obtained at concentration of 1 mg/ml, 500 µg/ml, 250 µg/ml, and 100 µg/ml respectively.
Statistical Analysis Used: Descriptive statistics was done to analysis the bacterial count in different concentration.
Results: The extracts obtained from Rosa gallica and psidium guajava L had a better anti-bacterial property when compared to the other medicinal plants and the extracts obtained from Rosa gallica and psidium guajava L had a better anti-fungal property when compared to the other two medicinal plants.
Conclusions: These plants extracts showed anti-bacterial property in previous studies to add on to the review of literature in our study it proved that Rosa gallica and psidium guajava L had a better anti-fungal activity when compared to another two medicinal plant extract. The clinical significance of the medicinal plant extract can be effectively used as anti-bacterial and anti-fungal agent as it was compared with the gold standard values of kanamycin and Ketoconazole.
Keywords: Anti-bacterial, anti-fungal, minimum inhibitory concentration and medicinal plant